Emerging tools for bioluminescence imaging
نویسندگان
چکیده
Bioluminescence (BL) relies on the enzymatic reaction between luciferase, a substrate conventionally named luciferin, and various cofactors. BL imaging has become widely used technique to interrogate gene expression cell fate, both in small large animal models of research. Recent developments include generation improved luciferase–luciferin systems for deeper more sensitive as well new caged luciferins report activity other intracellular functions. Here, we critically evaluate emerging tools aiming provide reader with an updated compendium latest (2018–2020) their notable applications.
منابع مشابه
Software Tools for the Analysis of Functional Magnetic Resonance Imaging
Functional magnetic resonance imaging (fMRI) has become the most popular method for imaging of brain functions. Currently, there is a large variety of software packages for the analysis of fMRI data, each providing many features for users. Since there is no single package that can provide all the necessary analyses for the fMRI data, it is helpful to know the features of each software package. ...
متن کاملEmerging Imaging and Genomic Tools for Developmental Systems Biology.
Animal development is a complex and dynamic process orchestrated by exquisitely timed cell lineage commitment, divisions, migration, and morphological changes at the single-cell level. In the past decade, extensive genetic, stem cell, and genomic studies provided crucial insights into molecular underpinnings and the functional importance of genetic pathways governing various cellular differenti...
متن کاملMolecular Imaging Using Bioluminescence
Recent progress in the development of non-invasive imaging technologies continues to strengthen the role of molecular imaging in biological research. Small animal imaging with bioluminescence has been validated recently in a variety of research models and have been shown to provide continuous quantitative monitoring of the location(s), magnitude, and time-variation of gene delivery and/or expre...
متن کاملCellular bioluminescence imaging.
Bioluminescence imaging of live cells has recently been recognized as an important alternative to fluorescence imaging. Fluorescent probes are much brighter than bioluminescent probes (luciferase enzymes) and, therefore, provide much better spatial and temporal resolution and much better contrast for delineating cell structure. However, with bioluminescence imaging there is virtually no backgro...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Current Opinion in Chemical Biology
سال: 2021
ISSN: ['1367-5931', '1879-0402']
DOI: https://doi.org/10.1016/j.cbpa.2021.02.005